Short Citation:
National Centers for Coastal Ocean Science, 2020: National Status and Trends: Contaminant body burdens and histopathology of fish and shellfish from Alaska, https://inport.nmfs.noaa.gov/inport/item/38941.

Item Identification

Title: National Status and Trends: Contaminant body burdens and histopathology of fish and shellfish from Alaska
Short Name: NPRB_2010-19
Status: Completed
Publication Date: 2011-06-15
Abstract:

In response to the growing concerns among Chugach communities, contaminant body burden and histopathological condition of chum and sockeye salmon (Oncorhynchus keta and Oncorhynchus nerka) and the shellfish cockles and softshell clams (Clinocardium nuttallii and Mya arenaria) were assessed. The fish and shellfish were collected from traditional subsistence harvest areas in the vicinity of Nanwalek, Port Graham and Seldovia, AK, and were analyzed for trace metals and residues of organic contaminants. Additionally, the fish and shellfish were histologically characterized for the presence, prevalence and severity of tissue pathology, disease, and parasite infections. Data served under this project include measurements of trace elements, polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls, DDTs, chlorinated hydrocarbons and histopathology parameters, which include an array of about 30 parasitic taxa (e.g. bucephalus, chlamydia, ciliates, cestodes and nematodes) and 11 diseases (e.g. tumors, neoplasm and necrosis). This project provides invaluable baseline data that is georeferenced and served on the internet through the NOAA's National Status and Trends data portal.

Purpose:

Specific objectives of the study were to: the health risks associated with consumption of subsistence food items collected in the traditional harvest ground in the Chugach Native communities of south-central Alaska. To achieve this goal the following objectives were accomplished: 1) Characterize the potential contamination of sessile (shellfish) vs. mobile (salmon) components of the subsistence fishery, using body burdens and histopathology as metrics; 2) Assess any potential correlations between body burdens and histopathology data; 3) Evaluation of the health condition of the fish with analyses of tissue pathogens and occurrences of diseases.

Notes:

998

Other Citation Details:

Apeti, D.A., S.I. Hartwell, W.E. Johnson and G.G. Lauenstein. 2012. National Status and Trends Bioeffects Progam: Field Methods. NOAA National Centers for Coastal Ocean Science, Center for Coastal Monitoring and Asssessment. NOAA NCCOS Technical Memorandum 135. Silver Spring, MD.27 pp. Apeti, D.A., W.E. Johnson, K.L. Kimbrough, and G.G. Lauenstein. 2012. National Status and Trends Mussel Watch Proram: Sampling Methods 2012 Update. NOAA Technical Memorandum 134. NOAA National Centers for Coastal Ocean Science, Center for Coastal Monitoring and Asssessment. Silver Spring, MD. 39 pp. Kim, Y., Ashton-Alcox, K.A. and Powell. E.N. 2006. Histological Techniques for Marine Bivalve Molluscs: Update. Silver Spring, MD. NOAA Technical Memorandum NOS NCCOS, 27. 76 pp. Meyers, T. R., and J. D. Hendricks. 1985. Histopathology. In G.M. Rand and S.R. Petrocelli (editors), Fundamentals of aquatic toxicology, p. 283-331. Hemisphere, Washington, DC. McDonald, S. J., D. S. Frank, J. A. Ramirez, B. Wang, and J. M. Brooks. 2006. Ancillary Methods of the National Status and Trends Program: Update 2000-2006, NOAA Technical Memorandum, NOS NCCOS 28. Lauenstein, G. G. and A. Y. Cantillo (1993) Sampling and analytical methods of the National Status and Trends Program National Benthic Surveillance and Mussel Watch Kimbrough K.L. and G.G. Lauenstein. 2006a. Organic Contaminant Analytical Methods of the National Status and Trends Program: 2000-2006. U.S. Dept. Comm. NOAA Technical Memorandum 30, NOS NCCOS, Silver Spring, MD. Kimbrough, K.L. and G.G. Lauenstein. 2006b. Trace Metal Analytical Methods of the National Status and Trends Program: 2000-2006. US Dept. Comm., NOAA Technical Memorandum 29, NOS NCCOS, Silver Spring, MD. Hartwell, S.I., A.D. Apeti,., L.W. Claflin, W.E. Johnson, and K.L. Kimbrough. 2009. Sediment Quality Triad Assessment in Kachemak Bay: Characterization of Soft Bottom Benthic Habitats and Contaminant Bioeffects Assessment. North Pacific Research Board Final Report 726, 138pp.

Supplemental Information:

PRINCIPAL INVESTIGATORS: Dennis Apeti, NOAA Ocean Service, National Status and Trends Program; S. Ian Hartwell, NOAA Ocean Service, National Status and Trends Program; Mark Myers, NOAA National Marine Fisheries Service, Northwest Fisheries Science Center; Jeff Hetrick, Chugach Regional Resource Commission, Alvtiiq Pride Shellfish Hatchery SAMPLE COLLECTION INVESTIGATORS: Dennis Apeti, NOAA Ocean Service, National Status and Trends Program; S. Ian Hartwell, NOAA Ocean Service, National Status and Trends Program; Mark Myers, NOAA National Marine Fisheries Service, Northwest Fisheries Science Center; Jeff Hetrick, Chugach Regional Resource Commission, Alvtiiq Pride Shellfish Hatchery SAMPLES PROCESSING INVESTIGATORS: -Fish necropsis: Dennis Apeti, NOAA Ocean Service, National Status and Trends Program, S. Ian Hartwell, NOAA Ocean Service, National Status and Trends Program; Mark Myers, NOAA National Marine Fisheries Service, Northwest Fisheries Science Center Necropsis was conducted at the NOAA/UAF Kasitsna laboratory. http://www.nurp.noaa.gov/Spotlight/KasitsnaBay.htm. -Contaminant analyses: Organic and inorganic chemicals in fish and shellfish tissues: TDI Brooks Inc., College Station, TX. http://ccma.nos.noaa.gov/about/coast/nsandt/download.aspx. -Histopatology characterization in fish: Mark Myers, NOAA National Marine Fisheries Service, Northwest Fisheries Science Center. http://www.nwfsc.noaa.gov/. -Histopathology characterization in shellfish: Rutgers University, Haskin Shellfish Research Laboratory. http://hsrl.rutgers.edu/

Keywords

Theme Keywords

Thesaurus Keyword
ISO 19115 Topic Category biota
ISO 19115 Topic Category environment
ISO 19115 Topic Category health
ISO 19115 Topic Category oceans
NOS Data Explorer Topic Category Environmental Monitoring
None analytes
None bivalve mollusk
None chemical
None coastal ecosystem
None coastal monitoring
None contaminants
None DDT
None estuary
None inorganic
None NCCOS Research Data Type > Field Observation
None NCCOS Research Priority > Long-term Monitoring
None NCCOS Research Priority > Stressors, Impacts, Mitigation, and Restoration (SIMR)
None NCCOS Research Topic > Bioeffects/Toxicity
None NCCOS Research Topic > Chemical Contaminants
None NCCOS Research Topic > Monitoring
None organic
None organochlorines
None PAH
None PCB
None pesticides
None polychlorinated biphenyls
None polynuclear aromatic hydrocarbons
None TBT
None total butyltins
None trace element

Spatial Keywords

Thesaurus Keyword
None Alaska
None Kachemak Bay
None Lower Cook Inlet
None Nanwalek
None NCCOS Research Location > Geographic Area > Coastal Ocean
None NCCOS Research Location > Region > Alaska
None NCCOS Research Location > U.S. States and Territories > Alaska
None Port Graham
None Seldovia
None Southcentral Alaska

Physical Location

Organization: National Centers for Coastal Ocean Science
City: Silver Spring
State/Province: MD

Data Set Information

Data Set Scope Code: Data Set
Maintenance Frequency: None Planned
Data Presentation Form: Table (digital)
Entity Attribute Overview:

The data dictionary may be found at: http://ccma.nos.noaa.gov/about/coast/nsandt/dictionaries/ba_mw_chemistry_dictionary.aspx

Entity Attribute Detail Citation:

National Status and Trends Program - reference data dictionary http://ccma.nos.noaa.gov/about/coast/nsandt/dictionaries/ba_mw_chemistry_dictionary.aspx

Distribution Liability:

These data were prepared by an agency of the United States Government. Neither the United States Government nor any agency thereof, nor any of their employees, make any warranty, expressed or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed in this report, or represents that its use would not infringe privately owned rights. Reference therein to any specific commercial product, process, or service by trade name, trademark, manufacturer, or otherwise does not necessarily constitute or imply its endorsement, recommendation, or favoring by the United States Government or any agency thereof. Any views and opinions of authors expressed herein do not necessarily state or reflect those of the United States Government or any agency thereof. Although all data have been used by NOAA, no warranty, expressed or implied, is made by NOAA as to the accuracy of the data and/or related materials. The act of distribution shall not constitute any such warranty, and no responsibility is assumed by NOAA in the use of these data or related materials.

Data Set Credit: The partners in the Resurrection Bay Interspecies Study were: (1) National Oceanographic and Atmospheric Administration (NOAA); (2) Chugach Regional Resource Commission(CRRC), (3) Alutiiq Pride Shellfish Hatchery.

Support Roles

Data Steward

CC ID: 451849
Date Effective From: 2011-06-15
Date Effective To:
Contact (Position): NCCOS Scientific Data Coordinator
Email Address: NCCOS.data@noaa.gov

Distributor

CC ID: 451851
Date Effective From: 2011-06-15
Date Effective To:
Contact (Position): NCCOS Scientific Data Coordinator
Email Address: NCCOS.data@noaa.gov

Metadata Contact

CC ID: 451852
Date Effective From: 2011-06-15
Date Effective To:
Contact (Position): NCCOS Scientific Data Coordinator
Email Address: NCCOS.data@noaa.gov

Point of Contact

CC ID: 451850
Date Effective From: 2011-06-15
Date Effective To:
Contact (Position): NCCOS Scientific Data Coordinator
Email Address: NCCOS.data@noaa.gov

Principal Investigator

CC ID: 451853
Date Effective From: 2011-06-15
Date Effective To:
Contact (Person): Piniak, Greg
Email Address: greg.piniak@noaa.gov

Extents

Currentness Reference: Ground Condition

Extent Group 1

Extent Group 1 / Geographic Area 1

CC ID: 451856
W° Bound: -151.9611
E° Bound: -151.6059
N° Bound: 59.487
S° Bound: 59.3386
Description

Intertidal coastal areas off Port Graham, Nanwalek and Seldovia in Lower Cook Inlet

Extent Group 1 / Time Frame 1

CC ID: 451855
Time Frame Type: Discrete
Start: 2010

Access Information

Security Class: Unclassified
Data Access Constraints:

None

Data Use Constraints:

NOAA requests that all individuals who download NOAA data acknowledge the source of these data in any reports, papers, or presentations. If you publish these data, please include a statement similar to: "Some or all of the data described in this article were produced by the NOAA's Ocean Service through its National Status and Trends Program (NSandT)"

Distribution Information

Distribution 1

CC ID: 451858
Download URL: https://products.coastalscience.noaa.gov/collections/ltmonitoring/nsandt/default.aspx
Description:

Downloadable Data

File Type: xml

Distribution 2

CC ID: 451857
Download URL: https://products.coastalscience.noaa.gov/collections/ltmonitoring/nsandt/default.aspx
Description:

Downloadable Data

File Type: txt

Activity Log

Activity Log 1

CC ID: 451900
Activity Date/Time: 2016-11-16
Description:

Date that the source FGDC record was last modified.

Activity Log 2

CC ID: 451899
Activity Date/Time: 2017-04-05
Description:

Converted from Content Standard for Digital Geospatial Metadata (version FGDC-STD-001-1998) using 'fgdc_to_inport_xml.pl' script. Contact Tyler Christensen (NOS) for details.

Activity Log 3

CC ID: 600450
Activity Date/Time: 2017-11-01
Description:

Replaced entire Lineage section to populate new Source Contribution field.

Data Quality

Accuracy:

MEASUREMENT QUALITY OBJECTIVES: Sample collection followed established protocols for the National Status and Trends Program. The measurement quality objectives of NSandT specify accuracy and precision requirements of 30% for organic analytes and 15% for inorganic analytes in tissue samples. QA procedures include running blanks, spiked samples, and standard reference materials with each batch of samples. Any batch failing to meet the specifications is reanalyzed or rejected. The QA Criteria may be found in NOAAs Tech Memo NOS NCCOS 30 and 29. The standard NSandT analytical protocols are described in Kimbrough et al. (2006a and 2006b). The histological analyses of shellfish were performed at Rutgers University's Haskin Shellfish Laboratory. A detailed account of the protocol is described in the NOAA's NOS/NCCOS technical memorandum number 27 (Kim et al. 2006). From each site 5 individual organisms of legal harvestable size (38 mm) were randomly selected and prepared for the analysis. The animal was placed in Davidson's fixative for 1 week and then transferred to 70% alcohol for storage. For all bivalves, the fixed tissue samples were embedded in paraffin after dehydration and clearing. The tissue-paraffin block was then placed in a freezer overnight before sectioning. The paraffin-embedded tissue blocks were first sliced at 20 micrometers to expose an entire tissue cross-section, and then sectioned at 5 micrometers. Tissue sections were deparaffinized and hydrated using a xylene-ethanol series. Following hydration, slides were stained in a pentachrome series, dehydrated in a series of acetic acid dips followed by acetone, cleared in xylene and mounted in Permount. Each slide was examined microscopically. Shellfish histopathology characterization consisted of quantifying infection intensity of parasites, the occurrence and extent of tissue pathologies, and the intensity of diseases all of which were determined using quantitative or semi-quantitative measures. Fish histopathology analysis of salmon was performed at the NOAA's Environmental Conservation of the Northwest Fisheries Science Center, NOAA Fisheries, in Seattle, Washington. Histopathologic diagnosis on fish liver, head and trunk kidney, and gill tissues. Sections of liver, head and trunk kidney (1cm in thickness) and two gill arches collected from individual salmon will be preserved in Davidson's fixative. Lesions and parasites in tissue sections were identified and classified according to the criteria specified in comparative pathology (Meyers and Hendricks, 1985; Cotran et al., 1999)

Completeness Report:

Data are believed to be complete

Conceptual Consistency:

Sample collection followed established protocols for the National Status and Trends Program. The analytical instruments were calibrated by standard laboratory procedures. In total, approximately 5% of all analyses were QC analyses. Processing quality was considered acceptable if the following criteria were met: blanks were less than three times the minimum detection limit; accuracy, as determined by analysis of reference materials, was within 30% for organic analytes and within 20% for inorganic analytes; and precision, as determined by replicate analyses, was within 30% for organic analytes and within 20% for inorganic analytes. Additional specifications and guidelines are presented in Valente and Strobel (1993). Contact the project manager for additional information on the Quality Assurance Project Plan

Lineage

Sources

Histological Techniques for Marine Bivalve Molluscs: Update

CC ID: 600453
Contact Name: Kim, Y., Ashton-Alcox, K.A., Powell. E.N.
Publish Date: 2006-01-01
Extent Type: Discrete
Extent Start Date/Time: 2006
Source Contribution:

Reference for histopathology chracterization standard operation procedure in shellfish | Source Geospatial Form: document | Type of Source Media: paper

Histopathology

CC ID: 600457
Contact Name: Meyers, T. R. and Hendricks, J. D.
Publish Date: 1985-01-01
Extent Type: Discrete
Extent Start Date/Time: 1985
Source Contribution:

Reference for histopathology chracterization standard operation procedure in fish | Source Geospatial Form: document | Type of Source Media: paper

Major and Trace Element Analytical Methods of the National Status and Trends Program: 2000-2006

CC ID: 600455
Contact Name: Kimbrough, K.L. and Lauenstein, G.G.
Publish Date: 2006-01-01
Extent Type: Range
Extent Start Date/Time: 2000
Extent End Date/Time: 2006
Citation URL: http://ccma.nos.noaa.gov/publications/nsandtmethods.pdf
Source Contribution:

Reference for standard operation procedure for organic contaminant analysis | Source Geospatial Form: document | Type of Source Media: online

National Status and Trends Bioeffects Program: Field Methods. NOAA National Centers for Coastal Ocean Science, Center for Coastal Monitoring and Assessment

CC ID: 600452
Contact Name: Apeti, D.A., Hartwell, S.I., Johnson, W.E., Lauenstein, G.G.
Publish Date: 2012-01-01
Source Contribution:

Reference for standard operation procedure for sample collection | Source Geospatial Form: document | Type of Source Media: paper

Organic Contaminant Analytical Methods of the National Status and Trends Program: 2000-2006

CC ID: 600454
Contact Name: Kimbrough K.L. and Lauenstein, G.G.
Publish Date: 2006-01-01
Extent Type: Range
Extent Start Date/Time: 2000
Extent End Date/Time: 2006
Citation URL: http://ccma.nos.noaa.gov/publications/organicsmethods.pdf
Source Contribution:

Reference for standard operation procedure for inorganic contaminants (heavy metals)analysis | Source Geospatial Form: document | Type of Source Media: online

Sampling and Analytical Methods of the National Status and Trends Program Mussel Watch Project: 1993-1996 Update

CC ID: 600456
Contact Name: Lauenstein, G.G., and Cantillo, A.Y.
Publish Date: 1998-01-01
Extent Type: Range
Extent Start Date/Time: 1993
Extent End Date/Time: 1996
Citation URL: http://ccma.nos.noaa.gov/publications/tm130.pdf
Source Contribution:

Reference for sampling standard operation procedure | Source Geospatial Form: document | Type of Source Media: paper

Sampling and Analytical Methods of the National Status and Trends Program Mussel Watch Project: 1993-1996 Update

CC ID: 600451
Contact Name: Lauenstein, G.G., and Cantillo, A.Y.
Publish Date: 1998-01-01
Extent Type: Range
Extent Start Date/Time: 1985
Extent End Date/Time: 2012
Source Contribution:

Sampling and analytical methods used in this project followed the National Status and Trends standard field and analytical methods | Source Geospatial Form: document | Type of Source Media: paper

Process Steps

Process Step 1

CC ID: 600458
Description:

Data Acquisition: The sample collection followed the standard Mussel Watch protocols. Sampling Objective: Fish (chum and sockeye salmon) and shellfish (softshell clams and cockles) were collected for the analysis of environmental contaminants and disease. Sampling Methods Summary: Sampling followed quality controlled and quality assured procedures of the NSandT and national Marine Fisheries Service (NMFS) for sample collection (Apeti et al., 2012; Cantillo and Lauenstein 1998; Meyers and Hendricks, 1985). The salmon and shellfish were collected at the traditional harvest grounds of Nanwalek, Port Graham and Seldovia. For clams and cockles, composites of 30 and 10 individual organisms were collected for the contamination and disease analyses respectively. For the salmon, the intent was to do inter-species comparison, but also investigated any gender differences. Thus, at each location, 10 (5 females and 5 males) specimen of each species were collected. Mollusks were not shucked in the field; cleaned samples were then packed on ice and shipped to the laboratory. Composite samples were prepared by homogenizing the soft parts. Fish necropsy occurred as soon after death of the salmon as possible. The fish were sorted by species and measured to assert proper age range. To prevent cross contamination during necropsy, four sets of dissection tools were used, each for the removal of fish muscle, liver, kidney and gill

Process Date/Time: 2010-01-01 00:00:00

Child Items

Rubric scores updated every 15m

Rubric Score Type Title
Entity (ENT) NPRB1019_Contaminant
Entity (ENT) NPRB1019_Fish_Histopath2
Entity (ENT) NPRB1019_Shellfish_Histopath

Catalog Details

Catalog Item ID: 38941
Metadata Record Created By: Tyler Christensen
Metadata Record Created: 2017-04-05 12:49+0000
Metadata Record Last Modified By: SysAdmin InPortAdmin
Metadata Record Last Modified: 2019-06-04 13:14+0000
Metadata Record Published: 2017-11-09
Owner Org: NCCOS
Metadata Publication Status: Published Externally
Do Not Publish?: N
Metadata Next Review Date: 2018-11-10