Short Citation:
Pacific Islands Fisheries Science Center, 2019: Calcification rates of crustose coralline algae (CCA) derived from Calcification Accretion Units (CAUs) deployed at coral reef sites in Batangas, Philippines from 2012 to 2015, https://inport.nmfs.noaa.gov/inport/item/45816.

Item Identification

Title: Calcification rates of crustose coralline algae (CCA) derived from Calcification Accretion Units (CAUs) deployed at coral reef sites in Batangas, Philippines from 2012 to 2015
Short Name: Philippines: CAUs
Status: Completed
Publication Date: 2017
Abstract:

Laboratory experiments reveal calcification rates of crustose coralline algae (CCA) are strongly correlated to seawater aragonite saturation state. Predictions of reduced coral calcification rates, due to ocean acidification, suggest that coral reef communities will undergo ecological phase shifts as calcifying organisms are negatively impacted by changing seawater chemistry. Calcification accretion units, or CAUs, are used by the NOAA Coral Reef Ecosystem Program (CREP) to assess the current effects of changes in seawater carbonate chemistry on calcification and accretion rates of calcareous and fleshy algae.

CAUs, constructed in-house by CREP, are composed of two 10 x 10 cm flat, square, gray PVC plates, stacked 1 cm apart, and are attached to the benthos by SCUBA divers using stainless steel threaded rods. Deployed on the seafloor for a period of time, calcareous organisms, primarily crustose coralline algae and encrusting corals, recruit to these plates and accrete/calcify carbonate skeletons over time. By measuring the change in weight of the CAUs, the reef carbonate accretion rate can be calculated for that time period.

The calcification rate data described here were collected by CREP from CAUs moored at fixed climate survey sites located on hard bottom shallow water (< 15 m) habitats in the Philippines, in accordance with protocols developed by Price et al. (2012). Climate sites were established by CREP to assess multiple features of the coral reef environment (in addition to the data described herein) from March 2012 to June 2015, and five CAUs were deployed at each survey site.

In conjunction with benthic community composition data (archived separately), these data serve as a baseline for detecting changes associated with changing seawater chemistry due to ocean acidification within coral reef environments. The data can be accessed online via the NOAA National Centers for Environmental Information (NCEI) Ocean Archive.

Purpose:

The Coral Reef Ecosystem Program (CREP) at NOAA Fisheries is conducting in-situ climate monitoring across the U.S. Pacific Islands Region. Climate monitoring provides a comprehensive view of climate change impacts on coral reef ecosystems and helps identify areas of resilience and vulnerability. The key indicators used to identify and monitor climate-driven trends include 1) thermal stress caused by changes in sea temperature, 2) ocean acidification resulting from changes in carbonate chemistry, and 3) ecological impacts by collecting data on coral growth rates and community structure to understand the impacts of thermal stress and ocean acidification on the ecosystem.

This particular dataset for the Philippines is part of a 3-year project ("Climate, Biodiversity and Fisheries in the Coral Triangle: Embracing the E in Ecosystem Approaches to Fisheries Management") implemented by CREP. This project was funded by NOAA's Coral Reef Conservation Program (CRCP) and the U.S. Agency for International Development (USAID) Regional Development Mission Asia (RDMA) as part of the U.S. Coral Triangle Initiative, with additional support from the Coral Triangle Support Partnership and USAID Philippines.

The goal of the project was to build on CREP's expertise to provide tools and information about climate change, ocean acidification, and their impacts on biodiversity and fisheries that could inform and be incorporated into an Ecosystem Approach to Fisheries Management (EAFM) for the Philippines. CREP worked with local governments, communities, and NGOs to build science capacity by establishing robust observing capabilities and providing hands-on training to initiate collection of climate science information for the Verde Island Passage in the Philippines that can be used toward adaptive EAFM.

Supplemental Information:

The NOAA Coral Reef Ecosystem Program (CREP) traveled to the Verde Island Passage region of the Philippines in 2012 to deploy moored oceanographic (subsurface temperature recorders [STRs]) and ecological (calcification accretion units [CAUs] and autonomous reef monitoring structures [ARMS]) instrumentation, to collect surface and bottom water samples, and to conduct benthic photo-quadrat surveys, which included the collection of benthic images; in 2013 to collect additional water samples; and again in 2015 to recover the instrumentation and to collect another round of water samples and benthic images. All activities were conducted by SCUBA divers at five locations in the municipalities of Mabini and Tingloy, including Batong Buhay, Koala Reserve Area, Arthur's Reef, Twin Rocks, and Batalang Bato. At each of the five locations, a shallow and deep survey site was established for a total of 10 survey sites.

Keywords

Theme Keywords

Thesaurus Keyword
CoRIS Discovery Thesaurus Numeric Data Sets > Calcification Rate
CoRIS Theme Thesaurus EARTH SCIENCE > Biosphere > Aquatic Habitat > Reef Habitat
CoRIS Theme Thesaurus EARTH SCIENCE > Biosphere > Vegetation > Algae > Algal Cover
CoRIS Theme Thesaurus EARTH SCIENCE > Biosphere > Vegetation > Algae > Algal Growth > Calcification Rate
CoRIS Theme Thesaurus EARTH SCIENCE > Biosphere > Vegetation > Algae > Calcareous Macroalgae
CoRIS Theme Thesaurus EARTH SCIENCE > Biosphere > Vegetation > Algae > Crustose Coralline Algae
CoRIS Theme Thesaurus EARTH SCIENCE > Biosphere > Vegetation > Algae > Encrusting Macroalgae
CoRIS Theme Thesaurus EARTH SCIENCE > Biosphere > Vegetation > Algae > Fleshy Macroalgae
CoRIS Theme Thesaurus EARTH SCIENCE > Biosphere > Vegetation > Algae > Reef Monitoring and Assessment
CoRIS Theme Thesaurus EARTH SCIENCE > Biosphere > Vegetation > Algae > Reef Monitoring and Assessment > Calcification Accretion Unit (CAU)
CoRIS Theme Thesaurus EARTH SCIENCE > Biosphere > Zoology > Corals > Reef Monitoring and Assessment
CoRIS Theme Thesaurus EARTH SCIENCE > Biosphere > Zoology > Corals > Reef Monitoring and Assessment > Baseline studies
CoRIS Theme Thesaurus EARTH SCIENCE > Biosphere > Zoology > Corals > Reef Monitoring and Assessment > In Situ Biological
CoRIS Theme Thesaurus EARTH SCIENCE > Oceans > Coastal Processes > Coral Reefs
CoRIS Theme Thesaurus EARTH SCIENCE > Oceans > Ocean Chemistry > Calcification
CoRIS Theme Thesaurus EARTH SCIENCE > Oceans > Ocean Chemistry > Carbonate Chemistry
CoRIS Theme Thesaurus EARTH SCIENCE > Oceans > Ocean Chemistry > Ocean Acidification
CRCP Project 483
CRCP Project Climate, Biodiversity and Fisheries in the Coral Triangle: Embracing the E in Ecosystem Approaches to Fisheries Management
ISO 19115 Topic Category 002
ISO 19115 Topic Category biota
NODC DATA TYPES THESAURUS CALCIFICATION
NODC Observation Types Thesaurus laboratory analyses
NODC Project Names Thesaurus Coral Reef Conservation Program
NODC Project Names Thesaurus CORAL REEF STUDIES
NODC Project Names Thesaurus Coral Triangle Initiative
NODC Submitting Institution Names Thesaurus US DOC; NOAA; NMFS; Pacific Islands Fisheries Science Center; Ecosystem Sciences Division; Coral Reef Ecosystem Program
None Calcification Plate
None Coral Reef Ecosystem Division
None Coral Reef Ecosystem Program
None CRED
None CREP
None Ocean Acidification
None Pacific Islands Fisheries Science Center
None PIFSC
None Settling Plate
None United States Agency for International Development
None USAID

Spatial Keywords

Thesaurus Keyword
CoRIS Place Thesaurus COUNTRY/TERRITORY > Philippines > Batangas > Arthur's Rock (13N120E0002)
CoRIS Place Thesaurus COUNTRY/TERRITORY > Philippines > Batangas > Mabini (13N120E0032)
CoRIS Place Thesaurus COUNTRY/TERRITORY > Philippines > Batangas > Tingloy (13N120E0009)
CoRIS Place Thesaurus COUNTRY/TERRITORY > Philippines > Batangas > Twin Rocks Sanctuary (13N120E0005)
CoRIS Place Thesaurus OCEAN BASIN > Pacific Ocean > South China Sea > Balayan Bay > Arthur's Rock (13N120E0002)
CoRIS Place Thesaurus OCEAN BASIN > Pacific Ocean > South China Sea > Balayan Bay > Mabini (13N120E0032)
CoRIS Place Thesaurus OCEAN BASIN > Pacific Ocean > South China Sea > Balayan Bay > Twin Rocks Sanctuary (13N120E0005)
CoRIS Place Thesaurus OCEAN BASIN > Pacific Ocean > South China Sea > Maricaban Island > Tingloy (13N120E0009)
NODC Sea Area Names Thesaurus NW Pacific (limit-180)
None Arthur's Reef
None Batalang Bato
None Batong Buhay
None Koala Reserve Area
None Philippines
None Twin Rocks
None Verde Island Passage

Physical Location

Organization: Pacific Islands Fisheries Science Center
City: Honolulu
State/Province: HI
Country: USA

Data Set Information

Data Set Scope Code: Data Set
Data Set Type: CSV Files
Maintenance Frequency: None Planned
Data Presentation Form: Table (digital)
Entity Attribute Detail URL: https://inport.nmfs.noaa.gov/inport/item/45941
Distribution Liability:

While every effort has been made to ensure that these data are accurate and reliable within the limits of the current state of the art, NOAA cannot assume liability for any damages caused by errors or omissions in the data, nor as a result of the failure of the data to function on a particular system. NOAA makes no warranty, expressed or implied, nor does the fact of distribution constitute such a warranty.

Data Set Credit: PIFSC Coral Reef Ecosystem Program and funded by the NOAA Coral Reef Conservation Program and the United States Agency for International Development
Instrument (Deprecated): Not applicable
Platform (Deprecated): Not applicable
Physical Collection / Fishing Gear (Deprecated): Calcification Accretion Units (CAUs) are composed of two 10 x 10 centimeter (cm) flat, square, gray PVC plates, stacked 1 cm apart and are deployed on individual stainless steel stakes driven into the benthic substrate. Calcareous organisms recruit to these plates and accrete/calcify carbonate skeletons over 2-3 year deployments.

Support Roles

Data Steward

CC ID: 565305
Date Effective From: 2017
Date Effective To:
Contact (Person): Young, Charles W
Address: 1845 Wasp Blvd.
Honolulu, HI 96818
USA
Email Address: charles.young@noaa.gov
Contact Instructions:

Email preferred

Data Steward

CC ID: 565303
Date Effective From: 2012
Date Effective To: 2016
Contact (Person): Misa, Paula WL
Address: 1845 Wasp Blvd.
Honolulu, HI 96818
USA
Email Address: paula.misa@noaa.gov
Phone: 808-725-5458
Contact Instructions:

Email preferred

Distributor

CC ID: 565304
Date Effective From: 2017
Date Effective To:
Contact (Organization): National Centers for Environmental Information - Silver Spring, Maryland (NCEI-MD)
Address: NOAA/NESDIS E/OC SSMC3, 4th Floor, 1351 East-West Highway
Silver Spring, MD 20910-3282
Phone: (301) 713-3277
Contact Instructions:

Email preferred

Distributor

CC ID: 565299
Date Effective From: 2012
Date Effective To: 2017
Contact (Person): Kanemura, Troy T
Address: 1845 Wasp Blvd.
Honolulu, HI 96818
USA
Email Address: troy.kanemura@noaa.gov
Phone: (808)725-5422
Contact Instructions:

Email preferred

Metadata Contact

CC ID: 565298
Date Effective From: 2012
Date Effective To:
Contact (Person): DesRochers, Annette M
Address: 1845 Wasp Blvd.
Honolulu, HI 96818
USA
Email Address: annette.desrochers@noaa.gov
Phone: (808)725-5461
Business Hours: 8 am - 5 pm
Contact Instructions:

Email preferred

Originator

CC ID: 565300
Date Effective From: 2012
Date Effective To:
Contact (Organization): Pacific Islands Fisheries Science Center (PIFSC)
Address: 1845 Wasp Blvd.
Honolulu, HI 96818
USA
Phone: 808-725-5300
URL: http://www.pifsc.noaa.gov
Business Hours: 8:00 a.m. - 4:30 p.m.

Originator

CC ID: 565306
Date Effective From: 2012
Date Effective To:
Contact (Organization): NOAA Coral Reef Conservation Program (CRCP) (CRCP)
Address: 1305 East West Highway 10th Floor
Silver Spring, MD 20910-3281
Phone: (301) 713-3155
URL: http://coralreef.noaa.gov

Point of Contact

CC ID: 565301
Date Effective From: 2015
Date Effective To:
Contact (Person): Oliver, Thomas
Address: 1845 Wasp Blvd.
Honolulu, HI 96818
USA
Email Address: thomas.oliver@noaa.gov
Phone: (808)725-5444
Contact Instructions:

Email preferred

Point of Contact

CC ID: 565302
Date Effective From: 2012
Date Effective To: 2014
Contact (Person): Young, Charles W
Address: 1845 Wasp Blvd.
Honolulu, HI 96818
USA
Email Address: charles.young@noaa.gov
Contact Instructions:

Email preferred

Extents

Currentness Reference: Ground Condition

Extent Group 1

Extent Description:

Mabini and Tingloy in Batangas, Philippines

Extent Group 1 / Geographic Area 1

CC ID: 566710
W° Bound: 120.87
E° Bound: 120.9
N° Bound: 13.728054
S° Bound: 13.658594
Description

Five survey locations in the municipalities of Mabini and Tingloy in Batangas, Philippines (near the Verde Island Passage), including Batong Buhay, Koala Reserve Area, Arthur's Reef, Twin Rocks, and Batalang Bato.

Extent Group 1 / Time Frame 1

CC ID: 566711
Time Frame Type: Range
Start: 2012-03-13
End: 2015-06-03
Alternate Start As Of Info: SBP1201 - deployed in 2012
Alternate End As Of Info: MP1507 - recovered in 2015
Description:

Time frame of the dataset from when the units were deployed in 2012 to when they were recovered in 2015.

Access Information

Security Class: Unclassified
Security Classification System:

Not applicable

Security Handling Description:

Not applicable

Data Access Policy:

Coral Reef Ecosystem Program (CREP) Data Sharing Recommendations, version 9.0 updated August 12, 2015:

CREP welcomes the opportunity to collaborate on research issues contributing to the scientific basis for better management of marine ecosystems. CREP has a very diverse set of field activities that generates large volumes of data using an array of data collection protocols.

The following recommendations are for your consideration as you use this data:

1) Data analyses should take all field exigencies into account. The most effective way to do this would be active collaboration with CREP principal investigators.

2) In all presentations, product releases, or publications using data generated by CREP, proper acknowledgement of both CREP and the individuals responsible for data collection is expected. Citing the DOI (if available) is preferred, a non-DOI example is listed below.

3) If you collect or generate data for the same study areas, CREP requests that you share relevant information on complimentary data collections.

4) Those receiving data are strongly urged to inform the CREP Data Management Team of any errors and discrepancies that are discovered during the course of using these data. They are further urged to bring to the attention of the Team all problems and difficulties encountered in using these data. This information is necessary in order to improve the collections and to facilitate more efficient and economical data processing and retrieval. The users are asked to supply copies of any missing data that may be located, and to provide information as to significant subsets and special aggregations of data that are developed in using the material provided.

Example citation:

"This publication makes use of data products provided by the Coral Reef Ecosystem Program (CREP), Pacific Islands Fisheries Science Center (PIFSC), National Marine Fisheries Service (NMFS), National Oceanic and Atmospheric Administration (NOAA), with funding support from the NOAA Coral Reef Conservation Program (CRCP) and the United States Agency for International Development. The analysis and interpretations presented here are solely that of the current authors”

Data Access Procedure:

Data can be accessed online via the NOAA National Centers for Environmental Information (NCEI) Ocean Archive.

Data Access Constraints:

None

Data Use Constraints:

Please cite NOAA Coral Reef Ecosystem Program (CREP) when using the data.

Suggested Citation:

Coral Reef Ecosystem Program; Pacific Islands Fisheries Science Center (2017). Calcification rates of crustose coralline algae (CCA) derived from Calcification Accretion Units (CAUs) deployed at coral reef sites in Batangas, Philippines from 2012 to 2015. NOAA's National Center for Environmental Information, https://inport.nmfs.noaa.gov/inport/item/45816.

Metadata Access Constraints:

None

Metadata Use Constraints:

None

Distribution Information

Distribution 1

CC ID: 565307
Download URL: http://accession.nodc.noaa.gov/0157633
Distributor: National Centers for Environmental Information - Silver Spring, Maryland (NCEI-MD) (2017 - Present)
File Name: CAU1_Assembly_Deployment_Recovery_CRED_SOP2015.pdf
Description:

NOAA Coral Reef Ecosystem Program (CREP) Calcification Accretion Unit (CAU) Assembly, Deployment, and Recovery Standard Operating Procedure (2015)

File Date/Time: 2015-09-10 00:00:00
File Type: PDF
File Size: 786 KB

Distribution 2

CC ID: 565308
Download URL: http://accession.nodc.noaa.gov/0157633
Distributor: National Centers for Environmental Information - Silver Spring, Maryland (NCEI-MD) (2017 - Present)
File Name: CAU2_Sample_Processing_CRED_SOP_2015.pdf
Description:

NOAA Coral Reef Ecosystem Program (CREP) Calcification Accretion Unit (CAU) Laboratory Analysis Standard Operating Procedure (2015)

File Date/Time: 2015-09-10 00:00:00
File Type: PDF
File Size: 811 KB

Distribution 3

CC ID: 565309
Download URL: http://accession.nodc.noaa.gov/0157633
Distributor: National Centers for Environmental Information - Silver Spring, Maryland (NCEI-MD) (2017 - Present)
File Name: PIFSC_DR-16-007_2015_SummaryReport_NMSAS.pdf
Description:

Summary report of baseline surveys and installations conducted in 2015 in the National Marine Sanctuary of American Samoa. This report includes the calcification accretion unit (CAU) methodology.

File Date/Time: 2016-04-26 00:00:00
File Type: PDF

Distribution 4

CC ID: 565310
Start Date: 2012-03-13
End Date: 2015-06-03
Download URL: http://accession.nodc.noaa.gov/0162831
Distributor: National Centers for Environmental Information - Silver Spring, Maryland (NCEI-MD) (2017 - Present)
File Name: V_CALCIFICATION_RATE_BY_UNIT_Philippines_2015.csv
Description:

Calcification rate data derived from calcification accretion units (CAUs) deployed and recovered by the NOAA Coral Reef Ecosystem Program at sites in the Philippines from 2012 to 2015.

File Date/Time: 2016-10-20 00:00:00
File Type: csv (comma-separated values)

URLs

URL 1

CC ID: 565311
URL: https://pifscblog.wordpress.com/2013/03/06/verde-island-passage/
URL Type:
Online Resource
File Resource Format: HTML
Description:

NOAA PIFSC Coral Reef Ecosystem Program blog post from the 2013 mission to the Philippines.

URL 2

CC ID: 565312
URL: https://www.pifsc.noaa.gov/cred/coral_triangle_initiative.php
URL Type:
Online Resource
File Resource Format: PHP
Description:

Information about the NOAA Coral Reef Ecosystem Program's activities in the Coral Triangle.

URL 3

CC ID: 565313
URL: http://www.pifsc.noaa.gov/cred/ocean_acidification.php
URL Type:
Online Resource
File Resource Format: PHP
Description:

Pacific Islands Fisheries Science Center, Coral Reef Ecosystem Program official website, Ocean Acidification page.

URL 4

CC ID: 565314
URL: http://www.pifsc.noaa.gov/cred/img/calcium_accretion_unit_after_two_years_sm.jpg
URL Type:
Browse Graphic
File Resource Format: JPEG
Description:

A CAU two years after deployment.

URL 5

CC ID: 565315
URL: https://www.pifsc.noaa.gov/library/pubs/DR-16-007.pdf
URL Type:
Online Resource
File Resource Format: PDF
Description:

Summary report of baseline surveys and installations conducted in 2015 in the National Marine Sanctuary of American Samoa. This report includes the calcification accretion unit (CAU) methodology.

Technical Environment

Description:

PIFSC Oracle database view: V_CALCIFICATION_RATE_BY_UNIT

Data Quality

Accuracy:

Prior to processing the calcification accretion unit (CAU) samples, laboratory analysts are trained to properly use relevant lab equipment (analytical balance, vacuum filtration pump, etc.) and are familiarized with the data entry tool/spreadsheet. Laboratory analysts are also required to go through the CAU Processing Standard Operating Procedure and other training materials.

Completeness Measure:

Calcification Accretion Units (CAUs) that have been deployed may not have been recovered due to logistical constraints of the following mission or could not be found when divers returned to the site. This can be quantified by comparing the number of recovered units to the number of units originally deployed (typically, 5 units per site).

Completeness Report:

The calcification rate and ratio of calcified materials to fleshy materials are determined for each Calcification Accretion Unit (CAU) recovered by the NOAA Coral Reef Ecosystem Program (CREP). Scripps Institution of Oceanography (Scripps) staff may conduct additional analyses of specific units to determine functional group coverage and to determine species recruitment rates based on the results of analyzed photos collected during the unit recovery and disassembly process.

Conceptual Consistency:

The sampling procedure is based on the protocols developed by Price et al. 2012 with slight modifications. Specifically, the following changes were implemented in 2013: 1) camera settings (adjusted to fit laboratory light conditions), and 2) use of freshwater in lieu of seawater during plate photo-documentation (seawater was not readily available in the new facility). Further, the processing procedures were also modified in 2013 such that the acceptable criteria for samples to be considered completely dry was set to 0.1 grams instead of 0.2 grams—that is, if two subsequent dry weights do not fall within the identified acceptable range (0.1 grams), the drying process will continue until this criteria is met.

Quality Control Procedures Employed:

Prior to processing a batch of CAUs recovered from a region, laboratory analysts inventory the recovered Calcification Accretion Units (CAUs) to ensure all units are accounted for and all serial numbers assigned to each unit match field data records. The Data Manager additionally performs quality control checks on the site deployment data and the recovery data for further accuracy. Once all CAUs from the region have been processed, the dataset is checked by the analysts to ensure: 1) all CAU plates have been processed, 2) there are no missing cell values, and 3) calculations for net weight of calcified materials are correct. The dataset is then turned over to the Data Manager for further quality control procedures and data ingestion into the NOAA Coral Reef Ecosystem Program's master database.

Data Management

Have Resources for Management of these Data Been Identified?: Yes
Approximate Percentage of Budget for these Data Devoted to Data Management: Unknown
Do these Data Comply with the Data Access Directive?: Yes
Is Access to the Data Limited Based on an Approved Waiver?: No
Approximate Delay Between Data Collection and Dissemination: Unknown
Actual or Planned Long-Term Data Archive Location: NCEI-MD
Approximate Delay Between Data Collection and Archiving: Unknown
How Will the Data Be Protected from Accidental or Malicious Modification or Deletion Prior to Receipt by the Archive?:

NOAA IRC and NOAA Fisheries ITS resources and assets.

Lineage

Lineage Statement:

Assembled calcification accretion units (CAUs) are attached to the benthos using stainless steel threaded rods. At each location (island) the NOAA Coral Reef Ecosystem Program (CREP) visits there are typically five CAUs deployed at each site. Calcareous organisms, primarily crustose coralline algae and encrusting corals, recruit to these CAUs and accrete/calcify carbonate skeletons over ~3-year deployments. Once recovered from the seafloor, the CAUs are processed to provide estimates of net calcification. CAUs have been deployed and replaced at existing, long-term monitoring sites during Pacific Reef Assessment and Monitoring Program (RAMP) cruises, and in the Coral Triangle, in accordance with protocols developed by Price et al. 2012.

Sources

NOAA Coral Reef Ecosystem Program (CREP) Calcification Accretion Unit (CAU) Assembly, Deployment, and Recovery Standard Operating Procedure (2015)

CC ID: 565319
Contact Type: Organization
Contact Name: Pacific Islands Fisheries Science Center, PIFSC
Publish Date: 2015-08-01
Citation URL: http://data.nodc.noaa.gov/coris/data/NOAA/nmfs/pifsc/cred/cau/CAU1_Assembly_Deployment_Recovery_CRED_SOP_2015.pdf

NOAA Coral Reef Ecosystem Program (CREP) Calcification Accretion Unit (CAU) Laboratory Analysis Standard Operating Procedure (2015)

CC ID: 565320
Contact Type: Organization
Contact Name: Pacific Islands Fisheries Science Center, PIFSC
Publish Date: 2015-09-01
Citation URL: http://data.nodc.noaa.gov/coris/data/NOAA/nmfs/pifsc/cred/cau/CAU2_Sample%20Processing_CRED_SOP_2015.pdf

Price NN, Martz TR, Brainard RE, Smith JE (2012) Diel Variability in Seawater pH Relates to Calcification and Benthic Community Structure on Coral Reefs. PLoS ONE 7(8): e43843. doi:10.1371/journal.pone.0043843

CC ID: 565321
Contact Name: Scripps Institution of Oceanography, University of California San Diego
Publish Date: 2012-08-28
Citation URL: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0043843

Process Steps

Process Step 1

CC ID: 565322
Description:

CALCIFICATION ACCRETION UNIT (CAU) ASSEMBLY, DEPLOYMENT and RECOVERY

CAUs are composed of two 10 x 10 centimeter (cm) flat, square, gray PVC plates, stacked 1 cm apart.

Five CAU units are deployed per site on individual stainless steel stakes driven into the substrate. The units are deployed in a clustered group with the spacing between each unit being 0.5 to 5 meters. CAU sites are situated at an average depth of 15 meters. Units are placed so the CAU plates are ~10 cm above the surface substrate and the top of the CAU plate is parallel to the surface of the water.

CAU units are recovered from the seafloor 2 to 3 years after deployment. Refer to the data files for the individual CAU site to determine the exact deployment time of a specific unit.

Refer to the NOAA Coral Reef Ecosystem Program (CREP) Calcification Accretion Unit (CAU) Assembly, Deployment, and Recovery Standard Operating Procedure (2015) for a detailed description of the protocols.

Process Date/Time: 2015-08-01 00:00:00
Process Contact: Young, Charles W
Email Address: charles.young@noaa.gov
Source: NOAA Coral Reef Ecosystem Program (CREP) Calcification Accretion Unit (CAU) Assembly, Deployment, and Recovery Standard Operating Procedure (2015)

Process Step 2

CC ID: 565323
Description:

CALCIFICATION ACCRETION UNIT (CAU) LABORATORY ANALYSIS

Once CAU units are recovered they are disassembled and each plate is rinsed with saltwater to remove loose sediment, sand and mobile fauna. The plates are photographed and individual images are captured of the Upper Plate, Top Side; Upper Plate, Bottom Side; Lower Plate, Top Side; Lower Plate, Bottom Side. These images are later analyzed to determine benthic composition on each plate surface. If pieces of calcified material fall off during the photographing process, the pieces are retained with the plate for the dissolution process.

After the plates have been photographed they are rinsed with fresh water and placed on a pre-weighed drying plate along with any dislodged pieces, and left to begin drying at room temperature for 24 hours and TO allow excess water to be removed. The drying plate assembly is then placed in the oven to dry at 60 degrees Celsius. If the difference in weights is less than 0.2 grams, the plates are considered dry and no further drying/weighing is required. If the difference in weights is greater than 0.2 grams the drying/reweighing process is repeated for as many 24-hour cycles as needed to obtain a difference of less than 0.2 grams. It should be noted that since the initial sample processing in 2012, the standard weighing protocol has since been improved such that the acceptable weight difference is 0.1 grams.

Once the plates are dry, each plate is placed in 5% Hydrocholoric Acid (HCl) solution to dissolve the calcium carbonate materials. The plate is scraped to remove all materials and rinsed with the 5% HCl solution. The plate is then placed in the oven at 60 degrees Celsius to dry for 24 hours before the dry plate is obtained. Through the entire dissolution process all 5% HCl rinse solution is preserved for later filtering.

The 5% HCl rinse process is repeated on all materials removed from the plate until the dissolution process has stopped and all calcium carbonate material has been removed. The fleshy material and used 5% HCl rinse materials are then separated using a vacuum filtration pump by pouring the solution into a Buchner funnel, mesh layer and pre-weighed filter paper. The filter paper and fleshy material are then dried and weighed to determine the weight of fleshy algae present on each plate. The weight of calcified materials on each plate is determined by difference, where the initial weight of the dried plate (prior to the dissolution process) minus the fleshy material weight equals the weight of carbonate material dissolved. The resulting calcification rates are reported in units of grams/area/time.

Refer to the NOAA Coral Reef Ecosystem Program (CREP) Calcification Accretion Unit (CAU) Laboratory Analysis Standard Operating Procedure (2015) for a detailed description of the protocols.

Process Date/Time: 2015-09-01 00:00:00
Process Contact: Young, Charles W
Email Address: charles.young@noaa.gov
Source: NOAA Coral Reef Ecosystem Program (CREP) Calcification Accretion Unit (CAU) Laboratory Analysis Standard Operating Procedure (2015)

Child Items

Rubric scores updated every 15m

Type Title
Entity (ENT) View: V_CALCIFICATION_RATE_BY_UNIT

Catalog Details

Catalog Item ID: 45816
Metadata Record Created By: Annette M DesRochers
Metadata Record Created: 2017-04-21 23:31+0000
Metadata Record Last Modified By: SysAdmin InPortAdmin
Metadata Record Last Modified: 2019-06-04 13:14+0000
Metadata Record Published: 2017-06-09
Owner Org: PIFSC
Metadata Publication Status: Published Externally
Do Not Publish?: N
Metadata Next Review Date: 2018-06-10